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J. Phys. IV France
Volume 11, Numéro PR10, Décembre 2001
Journées d'Étude des Équilibres entre Phases
Page(s) Pr10-233 - Pr10-235
Journées d'Étude des Équilibres entre Phases

J. Phys. IV France 11 (2001) Pr10-233-Pr10-235

DOI: 10.1051/jp4:20011037

Conformational study of HGV-NS3 (440-460) by circular dichroism (CD). Effect of lipophillic derivation on CD spectra

N. Rojo1, M.A. Alsina2 and I. Haro1

1  Department of Peptide & Protein Chemistry, IIQAB-CSIC, Jordi Girona 18-26, 08034 Barcelona, Spain
2  Department of Physicochemistry, Faculty of Pharmacy, University of Barcelona, Av. Joan XXIII, s/n, 08028 Barcelona, Spain

The present study was undertaken to examine the conformation by Circular Dichroism spectroscopy of the (440-460) : AIAYYRGKDSSIIKDGDLVVC peptide sequence belonging to the non-structural protein (NS3) of hepatitis G virus (HGV). The conformation of free HGV-NS3(440-460) and two lipophilic derivates at the N-terminus with palmitic and miristic acids respectively, was studied at different concentrations in HEPES as well as in the presence of the most commonly used agents for stabilizing conformations (TFE,HFIP).

© EDP Sciences 2001