J. Phys. IV France
Volume 104, March 2003
Page(s) 157 - 160

J. Phys. IV France
104 (2003) 157
DOI: 10.1051/jp4:200300052

Comparative studies of X-ray images and fluorescence images of the same specimens

T. Majima1, T. Tomie1 and H. Shimizu2

1  Photonics Research Institute and Life Electronics Laboratory, National Institute of Advanced Science and Technology (AIST), AIST Tsukaba Central 2, Umezono, Tsukuba, Ibaraki 305-8568, Japan
2  Engineering School, Kagawa University, Takamatsu, Kagawa 761-0396, Japan

A flash contact soft x-ray microscope using laser-induced plasma as a flash x-ray source is a practical instrument for observation of living organisms in water [1-4]. As previously reported we developed a tabletop flash contact soft x-ray microscope System [3]. In this System, x-ray images are given as whole projection of the specimens on the PMMA membrane. This causes us some complexity for understanding the x-ray images. It is necessary to attribute features in the x-ray images to sub-cellular structures of the specimen. For this purpose we have developed a new sample holder, where specimens are observable with a fluorescence microscope just before x-ray exposure. Fluorescence images of onion epidermal cells stained by DAPI and x-ray images of the same specimens are compared.

© EDP Sciences 2003