Soft X-ray contact microscopy and transmission electron microscopy: Comparative study of biological samples

T. Limongi; L. Palladino; E. Bernieri; G. Tomassetti; L. Reale; F. Flora; P. Cesare; C. Ercole; P. Aimola; A.M. Ragnelli

doi:doi:10.1051/jp4:200300096

Issue		J. Phys. IV France Volume 104, March 2003


Page(s)		345 - 348
DOI		https://doi.org/10.1051/jp4:200300096

J. Phys. IV France 104 (2003) 345
DOI: 10.1051/jp4:200300096

Soft X-ray contact microscopy and transmission electron microscopy: Comparative study of biological samples

T. Limongi¹, L. Palladino^{1, 2}, E. Bernieri³, G. Tomassetti¹, L. Reale¹, F. Flora⁴, P. Cesare⁵, C. Ercole⁵, P. Aimola⁵ and A.M. Ragnelli⁵

¹ Physics Department, L'Aquila University, Italy
² Prof. Libero Palladino, Physics Department, L'Aquila University, 67010 Coppito, L'Aquila, Italy
³ INFN, National Laboratory of Frascati, Italy
⁴ ENEA, Frascati, Italy
⁵ Basic and Applied Biology Department, L'Aquila University, Italy

Abstract
Isolated cellular organelles (mitochondria, chloroplasts) and cultured bacteria were analysed both by soft X-ray contact microscopy (SXCM), and by transmission electron microscopy (TEM) after negative staining. For each sample, a comparison was performed between images obtained with either technique, with the aim of facilitating the interpretation of SXCM images. The validity and the limits of this comparative approach are discussed.