Issue
J. Phys. IV France
Volume 04, Number C7, Juillet 1994
8th International Topical Meeting on Photoacoustic and Photothermal Phenomena
8 ITMP3 / 8éme conférence internationale de photoacoustique et photothermique
Page(s) C7-477 - C7-478
DOI https://doi.org/10.1051/jp4:19947111
8th International Topical Meeting on Photoacoustic and Photothermal Phenomena
8 ITMP3 / 8éme conférence internationale de photoacoustique et photothermique

J. Phys. IV France 04 (1994) C7-477-C7-478

DOI: 10.1051/jp4:19947111

Protein movement within the bacteriorhodopsin photocycle detected by beam deflection spectroscopy

P. Schulenberg, W. Gärtner and S.E. Braslavsky

Max-Planck-Institut für Strahlenchemie, Postfach 10 13 65, 45413 Mülheim an der Ruhr, Germany


Abstract
Photothermal beam deflection of the bacteriorhodopsin photocycle in the time range from 1 to 200 µs resolved a new process with a time constant of ca. 20 µs at 20 °C in addition to the decays of the K and L intermediates with time constants of 1.3 and 90 µs. The latter time constants are identical to those measured by flash photolysis with optical detection on the same samples. The additional process may be attributed to a protein conformational change concomitant with the proton transfer.



© EDP Sciences 1994