C. Chardonnet et G. Millot
J. Phys. IV France 135 (2006) 151-152
In-situ hybridization and single-molecule detectionE. Milhiet1, A. Débarre1, P. Tchénio1, J. Neveu2, D. Comas2 and T. Preat2
1 Laboratoire Aimé Cotton, Bât. 505, Campus d'Orsay, 91405 Orsay Cedex, France
2 DEPSN, Institut Alfred Fessard, Campus de Gif-sur-Yvette, 1 avenue de la Terrasse, 91190 Gif-sur-Yvette, France
(Published online 23 November 2006)
Fluorescence in-situ hybridization could be a powerful tool for analyzing the dynamics of memory phases if a semi-quantitative measurement of mRNA levels could be performed with a cellular resolution. While such a method does not yet exist, we show preliminary results in that direction where direct labelling of RNA probes with fluorescent dyes is combined with single-molecule-like detection.
© EDP Sciences 2006