J. Phys. IV France 127 (2005) 177-180
DOI: 10.1051/jp4:2005127027

Étude des dommages induits dans l'ADN par irradiation laser X-UV à 21.2 nm

K. Cassou¹, D. Ros¹, S. Kazamias¹, A. Klisnick¹, G. Jamelot¹, O. Guilbaud¹, B. Rus², M. Kozlová², J. Polan², A.R. Präg², M. Stupka², G. Eot-Houllier³, E. Sage³, M. Begusová⁴, V. Stísová⁴, M.-A.H. du Penhoat⁵, A. Touati⁵ and A. Chétioui<sup>5

1</sup>  Laboratoire d'Interaction du Rayonnement X avec la Matière, LIXAM, Bât. 350, CNRS UMR 8624, Campus de l'Université Paris-Sud XI, 91400 Orsay, France
²  Department of X-ray Laser/PALS Centre, Institute of Physics, Academy of Sciences of the Czech Republic, Na Slovance 2, 18221 Prague 8, Czech Republic
³  Laboratoire de Génotoxicologie et Cycle Cellulaire, Institut Curie, CNRS UMR 2027, Campus de l'Université Paris-Sud XI, 91400 Orsay, France
⁴  Nuclear Physics Institute, Academy of Sciences of the Czech Republic, Na Truhlárce 39/64, 18086 Prague 8, Czech Republic
⁵  Groupe de Physique des Solides, Universités Paris VI-VII, CNRS UMR 7588, CEA, France

Abstract
We report the preliminary result of the application of the collisional Ne-like soft x-ray laser as radiation source to induce DNA damage. The goal of this experiment was a test bed study of the damage yields induced by soft x-ray radiation in dried plasmid DNA sample. The saturated Ne-like soft x-ray laser available at the PALS facility, delivering several millijoules in a single 100 ps pulse at 21.2 nm was used to irradiate two different plasmids: pSP189 and pBS. The study is centered on the dose effect leading to single and double strand break in DNA.

© EDP Sciences 2005