J. Phys. IV France 125 (2005) 853-855
DOI: 10.1051/jp4:2005125197

Photoacoustic spectroscopy applied to the study of the influence of laser irradiation on corn seeds

C. Hernández Aguilar¹, A. Carballo C.², A. Cruz-Orea³, R. Ivanov⁴, E. San Martín Martínez⁵ and A. Michtchenko<sup>1

1</sup>  Instituto Politécnico Nacional, Sepi-Esime, Zacatenco, Unidad Profesional "Adolfo López Mateos", Col. Lindavista, CP 07738, Mexico, DF, Mexico
²  Colegio de Postgraduados, Programa de Semillas (IREGEP), Montecillo, Texcoco, Edo. de Mexico, CP 56180, Mexico
³  Departamento de Física, Centro de Investigación y de Estudios Avanzados-IPN, A.P. 14740. Mexico 07360, DF, Mexico
⁴  Unidad Académica de Física, Universidad Autónoma de Zacatecas, A.P. 580, Zacatecas, Zac., CP 98068, Mexico
⁵  Centro de Investigación en Ciencia Aplicada y Tecnología Avanzada-IPN, Legaria 694, Col. Irrigación, 11500 Mexico, DF, Mexico

Abstract
In the present study we were interested in the effects of low intensity laser irradiation on hybrid corn seeds CL₁ x CL₄ when these seeds were exposed to different laser intensities and irradiation times. In order to observe qualitative differences in chlorophyll a and b optical absorption spectra of seedling's leaves, whose seeds were irradiated and non irradiated, were obtained by using photoacoustic spectroscopy (PAS). A randomized complete blocks experimental design with three replications was used. The experimental unit included 10 seeds, from which we randomly choose three seedlings. The variance analysis (ANOVA) for both chlorophylls revealed significant (P < 0.05) differences among treatments

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