J. Phys. IV France
Volume 107, May 2003
Page(s) 569 - 572

J. Phys. IV France
107 (2003) 569
DOI: 10.1051/jp4:20030367

Evaluation of diatomea algae Thalassiosira weissflogii sensitivity to chloride mercury and methylmercury by chlorophyll fluorescence analysis

E.E. Graevskaya, T.K. Antal, D.N. Matorin, E.N. Voronova, S.I. Pogosyan and A.B. Rubin

Moscow State University, Biological Fac., 119899 Vorobjovy Gory, Moscow, Russia

Measurement of chlorophyll fluorescence has been shown to be a rapid, non-invasive, and reliable method to assess photosynthetic performance in a changing environment. In our study, the pulseamplitude-modulation (PAM) - fluorometric method was used to evaluate the sensitivity to chloride mercury and methylmercury chloride of diatomea microalgae Thalassiosira weissflogii. We found that 10-6 and  10-7 M MeHg led to a slow decrease in the PS II activity following for prolonged lag phase, whereas the algae was not sensitive to the same concentrations of HgCl 2. However observed PS II inactivation by methylmercury was not complete and about 10 percents ofthe cells kept the high level of PS II activity as it was shown by microfluorometric analysis. These cells could determine adaptation of algae to methylmercury effect. Both toxicants decreased the rate of PS II reparation, as well as increased a heat pathway of excitation dissipation in PS II antennae complex.

© EDP Sciences 2003