J. Phys. IV France 107 (2003) 565
Effect of methylmercury on the rat mast cell degranulationE.E. Graevskaya1, A. Yasutake2, R. Aramai2 and A.B. Rubin1
1 Moscow State University, Biological Fac., 119899 Vorobjovy Gory, Moscow, Russia
2 National Institute for Minamata Disease, 4058-18 Hama, Minamata, Kumamoto 867-0008, Japan
Methylmercury is the well-known neurotoxicant as weil as a modulator of the immune system. We investigated the effects of MeHg on the rat mast cell degranulation induced by nonimmunological stimuli (the selective liberator of histamine, compound 48/80, and calcium ionophore A23187) both in vivo and in vitro. In 8, 12 and 15 days afterthe final administration of MeHg we observed the suppression of calcium ionophore A23187-and 48/80-induced histamine release, which enhanced with time. In experiments in vitro incubation of peritoneal mast cells with MeHg alone in the dose range 10-8 to 10-6 did not induce mast cell degranulation, however modified the activation of mast cells by compound 48/80, and calcium ionophore A23187. We observed activation of stimulated secretion by preliminary incubation with low dose of MeHg 10-8 M and inhibition by dose of MeHg 10-6 M. These results show that MeHg treatment can modify mast cell function in vivo and in vitro and provide insight into the understanding what role this cell has in the pathogenesis of Minamata disease-comlected disorders.
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