J. Phys. IV France 107 (2003) 169
Coupled biosensor, biomimetic and chemometrics strategies for analysis of the metals in complex environmental matricesS.G. Bhand1, 2, E. Yilmaz3 and B. Danielsson1
1 Department of Pure and Applied Biochemistry, Lund University, Box 124, 22100 Lund, Sweden
2 Present address: Department of Chemistry, Birla Institute of Technology and Science, Pilani (Rajastan), Pin 333031, India
3 Department of Pure and Applied Biochemistry, Lund Institute of Technology, Box 124, 22100 Lund, Sweden
An analytical strategy to determine metals in environmental samples contaminated with both organophosphate pesticides and metals is presented. In the first step, parathion and arsenate were determined separately. Inhibition of the enzyme butyrylcholinesterase was quantified employing an enzyme thermistor and also with a CCD camera. Subsequently, parathion mixed with arsenate was measured. A molecularly imprinted polymer catalyst was then introduced as a pre-column to degrade OPs. The catalytic MIP enabled the measurement of metal from the mixture containing OPs. Hydrolysis of parathion, ethyl paraoxon, O-Ethyl-O- (4-nitrophenyl)- phenylphosphonothioate EPN and dichlorvos were monitored by using catalytic MIPs in the enzyme thermistor. Finally, inhibition patterns of parathion and arsenate were obtained employing multivariate analysis. The patterns generated after eliminating the OPs can be used for monitoring and mapping of metals in various environmental matrices.
© EDP Sciences 2003