J. Phys. IV France 104 (2003) 355
Three-dimensional visualization in projection X-ray microscopy of biological specimensH. Yoshimura, H. Maeda, A. Hori, T. Horikoshi, H. Takada and K. Igarashi
Department of Physics, School of Science and Technology, Meiji University, Higashimita 1-1-1, Tama-ku, Kawasaki 214-8571, Japan
We have developed a projection X-ray microscope by modifying a conventional scanning electron microscope (SEM). The operational process for optical alignment and focusing is as simple as with SEM. Because there are no X ray optical elements (e.g. a zone plate), this microscope can achieve a fairly deep focal depth. This implies it is possible to obtain projection images of thick and opaque sample in a single image without having to construct a composite image from thin sections. Taking this advantage. we inspected internal structure of thick biological specimens that were impossible to observe directly by either optical or electron microscopy.
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