J. Phys. IV France
Volume 104, March 2003
Page(s) 355 - 358

J. Phys. IV France
104 (2003) 355
DOI: 10.1051/jp4:200300099

Three-dimensional visualization in projection X-ray microscopy of biological specimens

H. Yoshimura, H. Maeda, A. Hori, T. Horikoshi, H. Takada and K. Igarashi

Department of Physics, School of Science and Technology, Meiji University, Higashimita 1-1-1, Tama-ku, Kawasaki 214-8571, Japan

We have developed a projection X-ray microscope by modifying a conventional scanning electron microscope (SEM). The operational process for optical alignment and focusing is as simple as with SEM. Because there are no X ray optical elements (e.g. a zone plate), this microscope can achieve a fairly deep focal depth. This implies it is possible to obtain projection images of thick and opaque sample in a single image without having to construct a composite image from thin sections. Taking this advantage. we inspected internal structure of thick biological specimens that were impossible to observe directly by either optical or electron microscopy.

© EDP Sciences 2003