J. Phys. IV France
Volume 104, March 2003
Page(s) 345 - 348

J. Phys. IV France
104 (2003) 345
DOI: 10.1051/jp4:200300096

Soft X-ray contact microscopy and transmission electron microscopy: Comparative study of biological samples

T. Limongi1, L. Palladino1, 2, E. Bernieri3, G. Tomassetti1, L. Reale1, F. Flora4, P. Cesare5, C. Ercole5, P. Aimola5 and A.M. Ragnelli5

1  Physics Department, L'Aquila University, Italy
2  Prof. Libero Palladino, Physics Department, L'Aquila University, 67010 Coppito, L'Aquila, Italy
3  INFN, National Laboratory of Frascati, Italy
4  ENEA, Frascati, Italy
5  Basic and Applied Biology Department, L'Aquila University, Italy

Isolated cellular organelles (mitochondria, chloroplasts) and cultured bacteria were analysed both by soft X-ray contact microscopy (SXCM), and by transmission electron microscopy (TEM) after negative staining. For each sample, a comparison was performed between images obtained with either technique, with the aim of facilitating the interpretation of SXCM images. The validity and the limits of this comparative approach are discussed.

© EDP Sciences 2003