J. Phys. IV France
Volume 104, March 2003
Page(s) 305 - 308

J. Phys. IV France
104 (2003) 305
DOI: 10.1051/jp4:200300086

Observation of E. colli cells by soft X-ray microscopy

S. Jiang1, 2, L. Chen2, H. Xie1, H. Gao1, J. Chen1, W.Q. Zhang1 and Z. Xu1

1  Shanghai Institute of Optics and Fine Mechanics, The Chinese Academy of Sciences, Shanghai 201800, China
2  National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei 230029, China

Employing X-rays from the synchrotron radiation facility of NSRL and contact imaging technique, hydrated, intact bacterial cells in their states, named by XL1-blue MRF', are studied. Some new information about the cells is acquired from their micrographs. The nucleiod containing DNA and proteins, and the mesosome has lower absorption to the X-ray with the wavelength 2.4 nm. And the ends of the bacterial cell are great different from each other in structure. The causes resulted in these interesting phenomena and its importance biologically need to be further studied.

© EDP Sciences 2003