J. Phys. IV France 104 (2003) 157
Comparative studies of X-ray images and fluorescence images of the same specimensT. Majima1, T. Tomie1 and H. Shimizu2
1 Photonics Research Institute and Life Electronics Laboratory, National Institute of Advanced Science and Technology (AIST), AIST Tsukaba Central 2, Umezono, Tsukuba, Ibaraki 305-8568, Japan
2 Engineering School, Kagawa University, Takamatsu, Kagawa 761-0396, Japan
A flash contact soft x-ray microscope using laser-induced plasma as a flash x-ray source is a practical instrument for observation of living organisms in water [1-4]. As previously reported we developed a tabletop flash contact soft x-ray microscope System . In this System, x-ray images are given as whole projection of the specimens on the PMMA membrane. This causes us some complexity for understanding the x-ray images. It is necessary to attribute features in the x-ray images to sub-cellular structures of the specimen. For this purpose we have developed a new sample holder, where specimens are observable with a fluorescence microscope just before x-ray exposure. Fluorescence images of onion epidermal cells stained by DAPI and x-ray images of the same specimens are compared.
© EDP Sciences 2003