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J. Phys. IV France
Volume 11, Numéro PR10, Décembre 2001
Journées d'Étude des Équilibres entre Phases
Page(s) Pr10-243 - Pr10-246
Journées d'Étude des Équilibres entre Phases

J. Phys. IV France 11 (2001) Pr10-243-Pr10-246

DOI: 10.1051/jp4:20011039

A view of three different lipid monolayers by atomic force microscopy

A. Ortiz, M.A. Alsina and Y. Cajal

Department of Physical Chemistry, Faculty of Pharmacy, University of Barcelona, Avn. Joan XXIII, s/n, 08028 Barcelona, Spain

Lipid monolayers prepared by the Langmuir technique are commonly used as model biomembranes. They have been extensively studied for years with a wide range of techniques, and during the last decade atomic force microscopy (AFM) [1] has emerged as a powerful tool for imaging the structure of the supported lipid films. AFM offers the view at nanometric scale of the transferred monolayers which provides information on the topographical organization of the films [2-41. In the present work we have studied the morphology of three lipid monolayers : zwitterionic DPPC, anionic DPPC : PI (9 : 1, mol : mol) and cationic DPPC : SA. (9 : 1, mol : mol). The structure of these three mixed monolayers transferred on mica is determined by AFM, and compared with the phase properties of the films at the air-water interface.

© EDP Sciences 2001