J. Phys. IV France 10 (2000) Pr7-283-Pr7-287
Incoherent elastic neutron scattering as a function of temperature : A fast way to characterise in-situ biological dynamics in complex solutionsG. Zaccai1, M. Tehei1, I. Scherbakova2, I. Serdyuk2, C. Gerez3 and C. Pfister1
1 Institut de Biologie Structurale, 41 avenue J. Horowitz, 38027 Grenoble cedex 1, France
2 Institute of Protein Research, 142292 Pushino, Moscow Region, Russia
3 CEA-Grenoble, DBMS/Chimie Biologique, 17 rue des Martyrs, 38054 Grenoble cedex 9, France
Thermal motions in macromolecules have been shown to be highly relevant in biology, allowing, for example, functionally important conformational changes to occur on much longer time-scales. Because of the values of their associated wavelengths and energies, thermal and cold neutrons are uniquely suitable for the characterization of these confined pico- to nano-second motions. Information useful to furthering understanding in biology, however, is mostly derived from measurements on many different systems under various conditions. Incoherent elastic neutron scattering temperature scans allow rapid screening of samples and conditions. The power of the method is demonstrated by recent results on proteins in solution and complex biological systems.
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