| Numéro |
J. Phys. IV France
Volume 7, Numéro C2, Avril 1997
Proceedings of the 9th International Conference on X-Ray Absorption Fine Structure
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|---|---|---|
| Page(s) | C2-629 - C2-630 | |
| DOI | https://doi.org/10.1051/jp4/1997121 | |
Proceedings of the 9th International Conference on X-Ray Absorption Fine Structure
J. Phys. IV France 7 (1997) C2-629-C2-630
DOI: 10.1051/jp4/1997121
1 Dip. di Medicina Sperimentale, Università dell' Aquila, 67100 Italy
2 Dip. di Scienze Biochimiche, Università di Roma "La Sapienza", 00185 Italy
3 LURE (Labs. CNRS, CEA, MEN), 91405 Orsay, France
4 Dip. di Fisica, Università di Roma "La Sapienza", 00185 Italy
© EDP Sciences 1997
J. Phys. IV France 7 (1997) C2-629-C2-630
DOI: 10.1051/jp4/1997121
Red Cell Aging : Fe-Heme Structure in Denatured Hemoglobins by XANES
S. Della Longa1, G. Amiconi2, I. Ascone3, A. Bertollini2, A. Bianconi4 and A. Congiu Castellano41 Dip. di Medicina Sperimentale, Università dell' Aquila, 67100 Italy
2 Dip. di Scienze Biochimiche, Università di Roma "La Sapienza", 00185 Italy
3 LURE (Labs. CNRS, CEA, MEN), 91405 Orsay, France
4 Dip. di Fisica, Università di Roma "La Sapienza", 00185 Italy
Abstract
The Fe-heme structure of hemoglobin under various 'in vitro' denaturing conditions has been investigated by Xray
Absorbption Near Edge Structure (XANES) spectroscopy at the Fe K-edge. The Fe3+ ion in denatured hemoglobin is found
both in low-spin and high-spin rhombic fields. New structural information on the low-spin forms (hemichromcf).
complementary to that already furnished by Electron Paramagnetic Resonance (EPR), is achieved. The presence and relevance
of these denatured forms in the 'in vivo' process of red cell aging is discussed.
© EDP Sciences 1997