Numéro
J. Phys. IV France
Volume 135, October 2006
Page(s) 151 - 152
DOI http://dx.doi.org/10.1051/jp4:2006135036
Publié en ligne 23 novembre 2006
Neuvième colloque sur les lasers et l'optique quantique
C. Chardonnet et G. Millot
J. Phys. IV France 135 (2006) 151-152

DOI: 10.1051/jp4:2006135036

In-situ hybridization and single-molecule detection

E. Milhiet1, A. Débarre1, P. Tchénio1, J. Neveu2, D. Comas2 and T. Preat2

1  Laboratoire Aimé Cotton, Bât. 505, Campus d'Orsay, 91405 Orsay Cedex, France
2  DEPSN, Institut Alfred Fessard, Campus de Gif-sur-Yvette, 1 avenue de la Terrasse, 91190 Gif-sur-Yvette, France


(Published online 23 November 2006)

Abstract
Fluorescence in-situ hybridization could be a powerful tool for analyzing the dynamics of memory phases if a semi-quantitative measurement of mRNA levels could be performed with a cellular resolution. While such a method does not yet exist, we show preliminary results in that direction where direct labelling of RNA probes with fluorescent dyes is combined with single-molecule-like detection.



© EDP Sciences 2006