J. Phys. IV France 11 (2001) Pr10-237-Pr10-241
Interaction of a hepatitis A synthetic antigen [105,109ABU]VP3(101-121) with lipid monolayersG. Monells1, A. Barceló1, I. Haro2, M.A. Alsina1 and M.A. Busquets1
1 Department of Physical Chemistry, School of Pharmacy, University of Barcelona, Avgda Joan XXIII, s/n, 08028 Barcelona, Spain
2 Department of Peptides, CID-CSIC, Jordi Girona 18-26, 08034 Barcelona, Spain
We describe the synthesis and characterization of an antigenic peptide : [105,109ABU]VP3(101-121), corresponding to the (101-121) sequence of VP3 protein from Hepatitis A virus. The physicochemical properties of the peptide were studied using lipid monolayers as a model membrane by means the Langmuir-Blodgett film technique. The main goal of the research was to get insight into the possible mechanism of action of the peptide at the membrane level and therefore, to better understand the proliferation and infection processes of the virus in the host. For that reason, interaction of the peptide with neutral, anionic and cationic phospholipids was studied by the determination of the penetration kinetics and compression isotherms. The peptide had surface activity, concentration dependent and was able to incorporate into lipid monolayers.
© EDP Sciences 2001