J. Phys. IV France 7 (1997) C2-629-C2-630
Red Cell Aging : Fe-Heme Structure in Denatured Hemoglobins by XANESS. Della Longa1, G. Amiconi2, I. Ascone3, A. Bertollini2, A. Bianconi4 and A. Congiu Castellano4
1 Dip. di Medicina Sperimentale, Università dell' Aquila, 67100 Italy
2 Dip. di Scienze Biochimiche, Università di Roma "La Sapienza", 00185 Italy
3 LURE (Labs. CNRS, CEA, MEN), 91405 Orsay, France
4 Dip. di Fisica, Università di Roma "La Sapienza", 00185 Italy
The Fe-heme structure of hemoglobin under various 'in vitro' denaturing conditions has been investigated by Xray Absorbption Near Edge Structure (XANES) spectroscopy at the Fe K-edge. The Fe3+ ion in denatured hemoglobin is found both in low-spin and high-spin rhombic fields. New structural information on the low-spin forms (hemichromcf). complementary to that already furnished by Electron Paramagnetic Resonance (EPR), is achieved. The presence and relevance of these denatured forms in the 'in vivo' process of red cell aging is discussed.
© EDP Sciences 1997