J. Phys. IV France 7 (1997) C2-83-C2-90
Mapping the Organic and Inorganic Components of Tissue Using NEXAFSC.J. Buckley1, N. Khaleque1, S.J. Bellamy1, M. Robins2 and X. Zhang3
1 Department of Physics, King's College London, Strand, London WC2R 2LS, UK
2 Department of Physiology, King's College London, Strand, London WC2R 2LS, UK
3 Department of Physics, State University of New York, Stony Brook, NY 11794, U.S.A.
A mapping technique which uses a scanning transmission soft X-ray microscope (STXM) is described. The technique has been developed and used to quantitatively map the calcium mineral and protein mass thicknesses in undemineralised, unstained, thin bone sections. Near complete femoral-neck sections of sibling normal and ovariectomised mice have been mapped. The results show the quantitative relationship between calcium and protein on the macro and microscopic scales for both tissues.
© EDP Sciences 1997