Numéro
J. Phys. IV France
Volume 03, Numéro C8, Décembre 1993
IX International Conference on Small Angle Scattering
Page(s) C8-253 - C8-256
DOI http://dx.doi.org/10.1051/jp4:1993849
IX International Conference on Small Angle Scattering

J. Phys. IV France 03 (1993) C8-253-C8-256

DOI: 10.1051/jp4:1993849

Structure of proteins unfolded by guanidinium chloride

P. CALMETTES1, D. DURAND1, J.C. SMITH2, M. DESMADRIL3, P. MINARD3 and R. DOUILLARD4

1  Laboratoire Léon Brillouin, CEA-CNRS, CE-Saclay, 91191 Gif-sur-Yvette cedex, France
2  Département de Biologie Cellulaire et Moléculaire, CE-Saclay, 91191 Gif-sur-Yvette cedex, France
3  Laboratoire d'Enzymologie Physico-Chimique et Moléculaire, Groupe de Recherche du CNRS, Université de Paris-Sud, 91405 Orsay, France
4  Laboratoire de Biochimie et Technologie des Protéines, Institut National de la Recherche Agronomique, BP. 527, 44026 Nantes cedex 03, France


Abstract
Small-angle neutron scattering measurements were performed on two very different proteins unfolded by guanidinium chloride. It is shown that the neutron spectra given by unfolded phosphoglycerate kinase and β-casein are similar to those of excluded volume homopolymer chains.



© EDP Sciences 1993