Numéro
J. Phys. IV France
Volume 03, Numéro C8, Décembre 1993
IX International Conference on Small Angle Scattering
Page(s) C8-233 - C8-236
DOI http://dx.doi.org/10.1051/jp4:1993844
IX International Conference on Small Angle Scattering

J. Phys. IV France 03 (1993) C8-233-C8-236

DOI: 10.1051/jp4:1993844

The in situ structure of the L3 and L4 proteins of the large subunit of E. coli ribosomes as determined by nuclear spin contrast variation

J. ZHAO and H.B. STUHRMANN

Abt. WS, GKSS-Research Center, 2054 Geesthacht, Germany


Abstract
Polarized Neutron scattering from dynamically polarized targets has been used to determine the in situ structure of the proteins L3 and L4 of the large subunit of E.coli ribosome. Both proteins (M≈2.3*104) have an elongated shape and radii of gyration of about 19 Å. The orientation of the proteins with respect to that of the ribosomal subunit has been determined. The distance between the centers of mass of L3 and L4 amounts to 125±10 Å.



© EDP Sciences 1993